ABSTRACT
A sindrome de Pallister Killian (SPK) e uma doenca rara caracterizada por multiplas malformacoes, retardo mental profundo e presenca de um isocromossomo...
Subject(s)
Humans , Female , Male , Child , Chromosome Aberrations/diagnosis , Fluorescein/analysis , In Situ Hybridization/methods , Abnormalities, Multiple/diagnosis , Chromosome Disorders/diagnosis , Chromosome Painting/methods , DNA Probes/analysis , Isochromosomes/geneticsABSTRACT
We present the clinical and laboratory features of 2 patients with B prolymphocitic leukemia. Both are females of the fith and seventh decade of life. One had the classical clinical picture of massive splenomegaly and a high white cell count, with characteristic prolymphocytes and the other was asymptomatic, with a low white cell count. The cells were positive to B cell lineage reagents with strong surface immunoglobuline (Ig) and unreactive to T cell antibodies. Analysis of Ig genes at the DNA level demonstrated that both cases had heavy-chain gene rearrangements, confirming the B-cell origin. These are the first patients of prolymphocytic leukemia described in Chile
Subject(s)
Middle Aged , Leukemia, Prolymphocytic/diagnosis , DNA Probes/analysis , Immunophenotyping/methods , Genetic Markers/immunologyABSTRACT
We have developed and tested a new way of typing Trypanosoma cruzi, mamely the use of cloned nuclear DNA fragments as genetic markers. Restriction fragment length polymorphisms were verified on Soutern blots hybridized to random probes. Fragment patterns were analyzed and dendrograms constructed. Our results on well characterized laboratory strains correlate well to published isoenzyme studies. Some of the probes were also hybridized to chromosomes separated by pulse field gel electrophoresis a higher degree of heterogeneity was observed at this level